Spawn Production Technology
Spawn is the vegetative mycelium from a selected mushroom grown on a convenient medium like wheat, pearl millet, sorghum, etc for raising mushroom crop. It essentially involves preparation of pure culture of mushroom from tissues/ spores that is generally maintained on any agar medium, followed by culturing on sterilized grains and further multiplied on grains. The spawn thus comprises of mycelium of the mushroom and a supporting medium which provides nutrition to the fungus during its growth.
Pure culture preparation Pure culture of fleshy fungi/mushrooms can be prepared either by multi-spore culture or tissue culture. Multi-spore culture is made from spore print that can be obtained by having a fresh fruit body after alcohol sterilization above a petriplate/sterilized paper. Millions of spores are collected within 48 hours. Serially diluted loop full of spores are then transferred to sterile Potato-dextrose-agar (PDA) or Malt-extract-agar culture slants. These slants are then incubated at 25˚C ± 2˚C for 2 weeks to obtain pure culture. For tissue culture, the basidiocarp after alcohol sterilization is cut longitudinally into 2 halves and bits from collar region are transferred to pre sterilized PDA or MEA culture medium. The Petri-plates are incubated at 25˚C ± 2˚C in BOD incubator for one week. Mycelium from growing edges is carefully transferred to MEA/PDA slants and again incubated for 2-3 weeks to obtain pure cultures
Substrate preparation Mushroom spawn can be prepared on any kind of cereal grains like wheat, jowar, bajra or rye and agricultural waste like corn cobs, wooden sticks, rice straw, saw dust and used tea leaves, etc. Spawn substrate i.e. cereal grains should be free from diseases and cereal grains should not be broken, old, and insect damaged. Most of the cereal grains are good substrate for spawn production
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