Culturing This step is necessary for anyone who wishes to have continuous growth of C. militaris. In this stage, cordyceps does not deviate much from the preferences of other commonly cultivated fungi and will grow on a variety of nutrient agars. Agars such as Malt extract agar (MEA) or potato dextrose agar (PDA) work great.However, C. militaris is susceptible to senescing (to deteriorate with age) fairly quickly. One way of prolonging your culture is to do less transfers. This means rather than growing out petri plates to culture again and subjecting the fungus to a multiple different cultures (think of having to move into a new house every week— it will be exhausting) it is better to make a lot of single transfer plates. If you are going to do transfers, make sure you are switching up it’s food source. Another way to prevent senescing is to do spore isolation work to isolate single ascospores for breeding. This requires more skill and equipment but it is worth it in the long run. If you do not want to do this yourself, there are a couple people in the U.S. that are started to do this work, including Ryan Paul Gates and Michael Weese.
Inoculam There are two typical ways to create inoculum in mushroom cultivation: grain spawn and liquid culture. Liquid culture has proven to be the more effective and practical choice of inoculum for customary C. militaris cultivation. Sterilized honey water is an excellent choice. Try using distilled or filtered water, and using organic honey.